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INFLAMMATION,
OXIDATIVE STRESS
AND
ANTIOXIDANTS |
MEMBERS AND
COLLABORATORS
Name |
Category |
E-mail |
Javier González Gallego (coord) |
CU
Fisiología ULE |
jgonga@unileon.es |
Sonia Sánchez Campos |
TU Fisiología ULE |
ssanc@unileon.es |
José A De Paz Fernández |
CU Fisiología ULE |
japazf@unileon.es |
Mar Almar Galiana
|
TU
Fisiología ULE |
mar.almar@unileon.es |
María J. Cuevas González |
TU Fisiología ULE |
mj.cuevas@unileon.es |
Ildefonso Alvear Ordenes |
AD Fisiología ULE |
ialvor@unileon.es |
Maria Victoria García Mediavilla |
AD Fisiología ULE |
mvgarm@unileon.es |
Susana Martínez Florez |
AD Anatomía ULE |
s.martinez@unileon.es |
Esther Nistal González |
PA Fisiología ULE |
esthernistal@hotmail.com |
Paula Rodríguez Míguelez |
Investigador
colaborador |
prodrm@unileon.es |
Rodrigo Fernández Gonzalo |
Investigador colaborador |
rodrigo.gonzalo@ki.se |
Brisamar Estébanez González |
Becario FPU |
b.estebanez@unileon.es |
David Porras Sanabria |
Becario JCyL |
dpors@unileon.es |
María Juárez Fernández |
Becario FPU |
mjuarf00@estudiantes.unileon.es |
Francisco Jorquera Plaza |
Jefe Servicio Aparato Digestivo CAULE |
fjorqueraplaza@gmail.com |
Fernando Ramos Ortega |
Jefe Sección Hematología Clínica CAULE |
framoso@aehh.org |
Isabel Fernández Natal |
Jefe Servicio Microbiología Clínica CAULE |
ifernandezn@saludcastillayleon.es |
Manuel Franco Benito |
Jefe
Servicio Oftalmología
CAULE |
mfrancobt@gmail.com |
Miguel Cordero Coma |
Coordinador Unidad Uveitis
CAULE |
miguelcorderocoma@gmail.com |
José M. García Ruiz de Morales |
Jefe Sección Inmunología CAULE |
jgarcir@gmail.com |
Octavio M. Rivero Lizcano |
Investigador
IECSCYL
CAULE |
orivero@saludcastillayleon.es |
OBJECTIVES
-
To
study effects of experimental and conventional therapies on
alterations in cell function.
-
To identify biochemical and functional markets useful for
diagnosis and prognosis in human pathologies.
-
To
investigate beneficial effects of exercise - from molecular
to organic levels - in different population groups.
ACTIVITIES
The group is
composed by researchers of different formation, including
biologists, physicians, veterinarians and pharmacists, who
come from the Department of Biomedical Sciences of the
University of Leon and from different Units of he Hospital of León. Their activity has been shaped in the
accomplishment of research projects funded by the European
Union, the Spanish National Plan of R+D, the Spanish Health
Research Fund or the Regional Government of Castilla y
León, and has made possible the development of research
contracts with diverse multinational companies of the
pharmaceutical sector. The group has
constituted one of the nodes of a Cooperative Research
Thematic Network of of the Carlos III Health Institute on
"Pathogenic mechanisms of viral hepatitis and steatohepatitis" (2003-2005). From its creation in 2006 they
are members of the “Hepatic and Digestive Diseases “CIBER of
the Spanish Health Ministry.
The group has centered its activity in
the study of the effects of reactive oxygen and nitrogen
species (free radicals, ROs and RNs) in diverse pathologies
(mainly hepatic diseases), and in situations in which body
function may be altered by the loss of the
oxidant/antioxidant balance (ej. aging, physical exercise).
Protective role of antioxidant molecules against ROs and RNs
induced alterations is also being investigated. The effect
of free radicals can be translated in a direct damage to
macro-molecules (lipids, proteins, ADN), but also in an
activation of transcription factors, such as NF-kappa B,
whose nuclear traslocation changes the expression of diverse
genes related to inflammatory processes (such as induced
nitric oxide sinthase, iNOS), oxidative damage, programmed
cellular death and others. All these aspects have been and
are being studied using different experimental models (cell
cultures, laboratory animals, research in humans).
In the area of exercise and physical
activity, research projects of applied character aimed to
improve physical fitness of diverse groups of population
(elderly, multiple sclerosis or chronic renal insufficiency
patients) are being developed.
METHODOLOGY
Studies:
Experimental and clinical.
In vitro
studies (isolation/culture of hepatic, endothelial and
muscle cells; cell line cultures).
In vivo
studies (laboratory animal studies, clinical studies in
patients and population groups).
Functional assessment of prescription of physical exercise.
Methodology:
Cell cultures.
Blood biochemistry.
Enzyme
activities and metabolic markers in blood and tissues
Markers of
inflammation and oxidative stress.
Markers of
muscle damage.
Markers of apoptosis.
Gene expression of inflammatory mediators,
antioxidant molecules and regeneration factors (Western
blot, RT-PCR, inmunohistochemistry).
Activation of nuclear
transcription factors.
Histopathological studies.
Anthropometric and nutritional assessment.
Neuromuscular assessment.
Ergospirométric
studies
and measurements of physical fitness.
TECHNOLOGICAL OFFER
-
Design and development of experimental and clinical research
programmes.
-
Advising and transfer of technology to companies in
the health sector
-
Assessment and prescription of physical exercise in
population groups with risk factors and related pathologies.
-
Development of physical exercise programmes fro the
improvement of health and physical fitness.
-
Training in laboratory techniques.
SELECTED PUBLICATIONS
-
Exercise
training modulates the gut microbiota profile and
impairs inflammatory signaling pathways in obese
children. R. Quiroga, E. Nistal, B. Estébanez, D.
Porras, M. Juárez-Fernández, S. Martínez-Flórez, M. V.
García-Mediavilla, J. A. de Paz, J. González-Gallego,
S.Sánchez-Campos, M. J. Cuevas. Experimental and
Molecular Medicine: 52(7), 1048-1061, 2020
-
Interplay between specific gut microbiota phylotypes
transplantation, diet and quercetin determines
obesity-related non-alcoholic fatty liver disease
(NAFLD) development in germ-free mice.
D. Porras, E. Nistal, S. Martínez-Flórez, J. L. Olcoz,
R. Jover, F.
Jorquera, J. González-Gallego, M. V.
García-Mediavilla y S. Sánchez-Campos.
Molecular Nutrition & Food Research:
63, e1800930, 2019
-
Melatonin modulates dysregulated circadian clocks in
mice with diethylnitrosamine-induced hepatocellular
carcinoma.
D. I. Sánchez, B. González-Fernández, I. Crespo, B.
San-Miguel, M. Álvarez, J. González-Gallego y M. J.
Tuñón.
Journal of Pineal Research:
e12506, 2018
-
Impact of resistance training on the
autophagy-inflammation-apoptosis crosstalk in elderly
subjects.
Y. Mejías-Peña, B. Estébanez, P. Rodriguez-Miguelez, R.
Fernandez-Gonzalo, M. Almar, J. A. de Paz, J.
González-Gallego y M. J. Cuevas.
Aging: 9, 408-418, 2017
-
Melatonin-induced increase in sensitivity of human
hepatocellular carcinoma cells to sorafenib is
associated with ROS production and mitophagy.
N. Prieto-Domínguez, R.
Ordóñez, A. Fernández, C.
Garcia-Ruiz, J. C. Fernandez-Checa, J. L. Mauriz y J.
González-Gallego.
Journal of Pineal Research:
61, 396-407, 2016
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Inhibition
of matrix metalloproteinase-9 and nuclear factor kappa B
contribute to melatonin prevention of motility and
invasiveness in HepG2 liver cancer cells. R. Ordoñez, S.
Carbajo-Pescador, N. Prieto-Dominguez, A. García-Palomo,
J. González-Gallego, J. L Mauriz. Journal of Pineal
Research: 56, 20-30, 2014
-
The human
liver fatty acid binding protein (FABP1) gene is
activated by FOXA1 and PPARalpha and repressed by C/EBPalpha:
Implications in FABP1 down-regulation in nonalcoholic
fatty liver disease. C. Guzmán, M. Benet, S. Pisonero-Vaquero,
M. Moya, M. V. García-Mediavilla, M. L.
Martínez-Chantar, et al. Biochimica Biophysica Acta:
1831, 803-818, 2013
CONTACT
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