NEUROBIOLOGY

 

 

MEMBERS AND COLLABORATORS

 

Nombre y apellidos

Cargo

E-mail

Arsenio Fernández López (Coord) CU Biología Celular ULE aferl@unileon.es

Pedro Calvo Fernández

CU Bioquímica ULE

pcalf@unileon.es

Carlos César Pérez García

TU Med. Veterinaria ULE

ccperg@unileon.es

Mª Ángeles Ríos Granja

TU Med. Veterinaria ULE

angeles.rios@unileon.es

Carlos Soria Gulina

Jefe Sección Anestesiología CAULE

dbccsg@unileon.es

Juan Carlos Bermejo

Facult. especializado. Anestesiologia CAULE

dbcjbg@unileon.es

Carlos Fernández López

TEU Ingeniería de Sistemas y Automática ULE

cferl@unileon.es

Irene Lorenzo Llorente

Becaria (financiada por Empresa a través de la ULE)

irene_atrida1@hotmail.com

 

 

OBJECTIVES

 

1. Estudio de los daños inducidos por isquemia cerebral o modelos in vitro sobre el sistema glutamatérgico

2. Estudio del efecto de los daños inducidos por isquemia cerebral o modelos equivalentes in vitro sobre el estrés de retículo y lo muerte celular por autofagia.

 

 

ACTIVITIES

 

The team is composed by researchers with different background, including biologists, physicians, and veterinarians from several Departments of the University and Hospital of León, Spain. This team is or has been supported by international, national and autonomic community projects as well as contracts or donations of different firms (Laboratorios Esteve S.A., Lipopharma SL, Covidien, etc.).

In addition to is activity research, this team is involved in formative activities through supervision of doctoral thesis, Doctorate and master programs.

 The team also performs research dissemination in an attempt to bring the society closer to the stroke problems and prevention through its web page http://neurobio.unileon.es/ictus and courses of laboratory techniques.

 The main line of research is based on the study of the cell vulnerability to stroke. In this line, the roles of the glutamatergic system, autophagy and reticulum stress are analyzed. The use of lipid therapies addressed to decrease the stroke damage has been started recently. Studies are performed in both in vivo and ex vivo models of stroke.

 The team has a wide experience in the characterization of biochemical markers related with stroke related neural damage.

 

METHODS

 

Types of studies

·         ex vivo studies (sections of hippocampus and cerebral cortex).

·         in vivo studies (global, focal and photo thrombotic models of cerebral ischemia).

Analytical methods

·         Location and quantification of biochemical markers using antibodies.

·         Measurement of ARNm levels using real time PCR (qPCR).

·         Hematology, blood biochemistry and urinalysis.

·         Blood pressure, electrocardiography, magnetic resonance imaging, laser-doppler and echocardiography.

 

 

TECHNOLOGICAL OFFER

 

1. Techniques based on the use of antibodies (Western blot, immunocytochemistry, obtaining polyclonal antibodies)

2. Molecular biology techniques (PCR, recombinant protein expression in bacteria)

3. Experimental models of stroke for drug screening

 

 

REPRESENTATIVE PUBLICATIONS

  • GABA(A) receptor chloride channels are involved in the neuroprotective role of GABA following oxygen and glucose deprivation in the rat cerebral cortex but not in the hippocampus. Llorente IL, Perez-Rodriguez D, Martínez-Villayandre B, Dos-Anjos S, Darlison MG, Poole AV, Fernández-López A. Brain Res. 2013;1533:141-51.

  • Unfolded protein response to global ischemia following 48 h of reperfusion in the rat brain: the effect of age and meloxicam. Llorente IL, Burgin TC, Pérez-Rodríguez D, Martínez-Villayandre B, Pérez-García CC, Fernández-López A. J Neurochem. 2013;127(5):701-10.

  • Age and meloxicam modify the response of the glutamate vesicular transporters (VGLUTs) after transient global cerebral ischemia in the rat brain. Llorente IL, Pérez-Rodríguez D, Burgin TC, Gonzalo-Orden JM, Martínez-Villayandre B, Fernández-López A. Brain Res Bull. 2013;94:90-7.

  • Differential effect of transient global ischaemia on the levels of γ-aminobutyric acid type A (GABA(A)) receptor subunit mRNAs in young and older rats. Montori S, Dos Anjos S, Poole A, Regueiro-Purriños MM, Llorente IL, Darlison MG, Fernández-López A, Martínez-Villayandre B. Neuropathol Appl Neurobiol. 2012;38(7):710-22.

  • Age-dependent modifications in the mRNA levels of the rat excitatory amino acid transporters (EAATs) at 48hour reperfusion following global ischemia. Montori S, Martínez-Villayandre B, Dos-Anjos S, Llorente IL, Burgin TC, Fernández-López A. Brain Res. 2010 Oct 28;1358:11-9.

  • AMPA receptor downregulation induced by ischaemia/reperfusion is attenuated by age and blocked by meloxicam. Montori S, Dos Anjos S, Ríos-Granja MA, Pérez-García CC, Fernández-López A, Martínez-Villayandre B. Neuropathol Appl Neurobiol. 2010;36(5):436-47.

  • Age and meloxicam attenuate the ischemia/reperfusion-induced down-regulation in the NMDA receptor genes. Montori S, Dos-Anjos S, Martínez-Villayandre B, Regueiro-Purriños MM, Gonzalo-Orden JM, Ruano D, Fernández-López A. Neurochem Int. 2010;56(8):878-85.

RELATED URLs

 

 http://neurobio.unileon.es

 

 

CONTACT: