|
|
NEUROBIOLOGY |
MEMBERS
AND COLLABORATORS
Nombre y apellidos |
Cargo |
E-mail |
Arsenio Fernández López
(Coord) |
CU Biología Celular ULE |
aferl@unileon.es |
Pedro Calvo Fernández |
CU
Bioquímica ULE |
pcalf@unileon.es |
Carlos César Pérez García |
TU
Med. Veterinaria ULE |
ccperg@unileon.es |
Mª
Ángeles Ríos Granja |
TU
Med. Veterinaria ULE |
angeles.rios@unileon.es |
Carlos Soria Gulina |
Jefe Sección Anestesiología
CAULE |
dbccsg@unileon.es |
Juan Carlos Bermejo |
Facult. especializado. Anestesiologia CAULE |
dbcjbg@unileon.es |
Carlos Fernández López |
TEU Ingeniería de Sistemas y Automática ULE |
cferl@unileon.es |
Irene Lorenzo Llorente |
Becaria (financiada por Empresa a través de la ULE) |
irene_atrida1@hotmail.com |
OBJECTIVES
1.
Estudio de los daños inducidos por isquemia cerebral o modelos in
vitro sobre el sistema glutamatérgico |
2.
Estudio del efecto de los daños inducidos por isquemia cerebral o
modelos equivalentes in vitro sobre el estrés de retículo y lo
muerte celular por autofagia. |
ACTIVITIES
The team is composed by researchers with different
background, including biologists, physicians, and
veterinarians from several Departments of the University and
Hospital of León, Spain. This team is or has been supported
by international, national and autonomic community projects
as well as contracts or donations of different firms (Laboratorios
Esteve S.A., Lipopharma SL, Covidien, etc.).
In addition to is activity research, this team is involved
in formative activities through supervision of doctoral
thesis, Doctorate and master programs.
The team also performs research dissemination in an attempt
to bring the society closer to the stroke problems and
prevention through its web page http://neurobio.unileon.es/ictus
and courses of laboratory techniques.
The main line of research is based on the study of the cell
vulnerability to stroke. In this line, the roles of the
glutamatergic system, autophagy and reticulum stress are
analyzed. The use of lipid therapies addressed to decrease
the stroke damage has been started recently. Studies are
performed in both in vivo and ex vivo models
of stroke.
The team has a wide experience in the characterization of
biochemical markers related with stroke related neural
damage.
METHODS
Types of studies
·
ex vivo
studies (sections of hippocampus and cerebral cortex).
·
in vivo
studies (global, focal and photo thrombotic models of
cerebral ischemia).
Analytical methods
·
Location and quantification of biochemical markers using
antibodies.
·
Measurement of ARNm levels using real time PCR (qPCR).
·
Hematology, blood biochemistry and urinalysis.
·
Blood pressure, electrocardiography, magnetic resonance
imaging, laser-doppler and echocardiography.
TECHNOLOGICAL OFFER
1. Techniques based on the use of antibodies (Western blot,
immunocytochemistry, obtaining polyclonal antibodies) |
2. Molecular biology techniques (PCR, recombinant protein expression
in bacteria) |
3. Experimental models of stroke for drug screening |
REPRESENTATIVE PUBLICATIONS
-
GABA(A) receptor chloride channels are involved in the
neuroprotective role of GABA following oxygen and
glucose deprivation in the rat cerebral cortex but not
in the hippocampus.
Llorente IL, Perez-Rodriguez D, Martínez-Villayandre B,
Dos-Anjos S, Darlison MG, Poole AV, Fernández-López A.
Brain Res. 2013;1533:141-51.
-
Unfolded protein response to global ischemia following
48 h of reperfusion in the rat brain: the effect of age
and meloxicam.
Llorente IL, Burgin TC, Pérez-Rodríguez D, Martínez-Villayandre
B, Pérez-García CC, Fernández-López A. J Neurochem.
2013;127(5):701-10.
-
Age and meloxicam modify the response of the glutamate
vesicular transporters (VGLUTs) after transient global
cerebral ischemia in the rat brain.
Llorente IL, Pérez-Rodríguez D, Burgin TC, Gonzalo-Orden
JM, Martínez-Villayandre B, Fernández-López A.
Brain Res Bull. 2013;94:90-7.
-
Differential effect of transient global ischaemia on the
levels of
γ-aminobutyric
acid type A (GABA(A)) receptor subunit mRNAs in young
and older rats.
Montori S, Dos Anjos S, Poole A, Regueiro-Purriños MM,
Llorente IL, Darlison MG, Fernández-López A, Martínez-Villayandre
B. Neuropathol Appl Neurobiol.
2012;38(7):710-22.
-
Age-dependent modifications in the mRNA levels of the
rat excitatory amino acid transporters (EAATs) at 48hour
reperfusion following global ischemia. Montori S,
Martínez-Villayandre B, Dos-Anjos S, Llorente IL, Burgin
TC, Fernández-López A. Brain Res. 2010 Oct 28;1358:11-9.
-
AMPA receptor downregulation induced by ischaemia/reperfusion
is attenuated by age and blocked by meloxicam.
Montori S, Dos Anjos S, Ríos-Granja MA, Pérez-García CC,
Fernández-López A, Martínez-Villayandre B. Neuropathol
Appl Neurobiol.
2010;36(5):436-47.
-
Age and meloxicam attenuate the
ischemia/reperfusion-induced down-regulation in the NMDA
receptor genes.
Montori S, Dos-Anjos S, Martínez-Villayandre B, Regueiro-Purriños
MM, Gonzalo-Orden JM, Ruano D, Fernández-López A.
Neurochem Int. 2010;56(8):878-85.
RELATED URLs
http://neurobio.unileon.es
CONTACT:
|