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CELL THERAPY IN OSTEOARTICULAR
DISEASES |
MIEMBERS AND COLLABORATORS
Name |
Category |
E-mail |
Vega Villa Suarez (coord.) |
CD
Anatomía y Anatomía Patológica Comparadas, Unileon |
vega.villar@unileon.es |
Julio G. Prieto
Fernández |
CU Fisiología Unileon,
|
julio. prieto@unileon.es |
Jaime Sánchez Lázaro |
Especialista, Caule |
jasanlazaro@gmail.com |
Jesús C. Betegón Nicolas |
Especialista, Caule |
betedoc@yahoo.es |
Luis Díaz Gallego |
Jefe de Sección. Caule |
ldg511@telefonica.net |
Rebeca Real Fernández |
Investigadora Colaboradora
|
dfirrf@unileon.es |
Marisa González Fernández |
Investigad.
Colaboradora |
marisagf@hotmail.com |
Daniel González Arabio |
Especialista, Caule |
danielarabio@yahoo.es |
Prudencio Alonso Otero |
Investigador
Colaborador |
prudencio.alonsootero@gmail.com |
Manuel La Nuez García |
Investigador Colaborador |
mlanuezgarcia@gmail.com |
José García Cosamalón |
Investigador colaborador |
jcosamalon@smonica.com |
OBJECTIVES
1. Use of mesenquimal stem cells ((MECs) for the
induction of chondrogenesis and development of
viable and efficient alternatives for culturing and
proliferation |
2. Study of the protective effects of the use of
hyaluronic acid (AH) and other Sysadoas in bone
pathology |
3. Physicochemical properties of the AH´s and
mechanisms of action of AH´s, Sysadoas and natural
products |
4. ABC transporters as efflux pumps of drugs
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ACTIVITIES
The group is formed by researchers of diverse background,
including biologist and physicians from several Departments
of the University of Leon and the Traumatology Unit of
Hospital of Leon
The first line of research tries to apply the knowledge from
chondrocytes to the MSCs. Results from these studies have
been presented as a patent application entitles
“Establishment of a new cell line called VELI for the in
vitro culture of Cryptosporidium parvum”.
The research about the application of Hyaluronic Acid is
based on the development of experimental models of arthrosis
and the studies of improvement of arthrosic pathologies as a
consequence of its intraarticular administration. In
addition, backbone surgery models in rabbit have been
developed, in order to study the potential differential
antifibrotic effect of several Hyaluronic Acids of variable
molecular weights. The change in the physicochemical
properties of several Hyaluronic Acids in in vitro assays is
simultaneously studied.
An additional line of research in the Unit has been
developed from several years in collaboration with
researchers of the Instituto de Sanidad Animal (INDEGSAL) of
the University of Leon related to the activity of the
transporters of the ABC (ATP-binding cassette) family. Their
role as active transporters or efflux pumps through the
plasma membrane of diverse compounds (such as endogen
metabolites, drugs, xenotoxins and carcinogen agents)
revealed their protective function due to their effect on
the systemic exposure and tissue penetration of some of
their substrates. These transporters also play a relevant
role in the phenomenom of multidrug resistance to
antitumoral drugs, and their induced expression decreases
the entry of antitumoral drugs inside the cells, reducing
the efficacy of some treatments.
METHODOLOGY
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To obtain
MSCs from the rabbit bone marrow and in vitro culture in static and dynamic
conditions
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To obtain
the fractions of cartilage extracellular matrix
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Generation
of experimental models for the development of arthrosis in the rabbit and/or
rat as a consequence of the breaking of the crossed ligament of the knee
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Determination of apoptosis and necrosis of chondrocytes by flow cytometry
and/or Tunnel method
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Quantification of nitrite and nitrate levels and properties of concentration
and molecular weight of hyaluronic acid by HPLC
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Study of
drug in vitro inhibitory capacity of ABC transporters (ABCG2) with its
relevance on the pharmacokinetics and excretion into milk of substrates.
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In vitro
characterization of substrates of the main ABC transporters in different
localizations
TECHNOLOGICAL OFFER
1. Development of animal models for the in vivo
study as complements of in vitro studies of
viscoelastic substances |
2. Advice and technology transfer to companies that
commercialize with viscoelastic substances |
3. Identification of drugs (human or veterinary
therapeutics) as substrates or inhibitors of the ABC
transporters |
REPRESENTATIVE PUBLICATIONS
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Antioxidant
profile of hyaluronan: physico-chemical features and its
role in pathologies.
G. Mendoza, J. G. Prieto, R. Rea, M. Pérez, G. Merino, A. I. Alvarez.
Mini Reviews in Medicinal Chemistry: 9, 1479-1488, 2009
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Reevaluation
of the roles of ABCG2 in the disposition of genistein. A. I.
Alvarez, F. Vallejo, B. Barrera, J. G. Prieto, F. Tomás Barberán, J. C.
Espín. Drug Metabolism and Disposition: 40, 2219, 2012
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The
bovine ATP-binding cassette transporter ABCG2 Tyr581Ser
single-nucleotide polymorphism increases milksecretion of the
fluoroquinolone danofloxacin. J. A. Otero, R. Real. A. de la
Fuente J. G. Prieto, M. Marqués, A. I. Alvarez, G. Merino. Drug
Metabolism and Disposition: 41, 546-549, 2013
CONTACT
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